Description:Mouse monoclonal antibody to human Interferon-gamma (IFN-γ)

Purification: Protein G affinity purified

Target Protein: HumanIFN-γ

Immunogen: Purified recombinant humanIFN-γ

Fusion Myeloma: Sp2/0-Ag14

Specificity:This antibody recognizes both recombinant and native human IFN-γ.

Species Reactivity: Human, other species not tested

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20oC

Research Area:Pro-inflammatory cytokine, anti-tumour and anti-viral immunity

Background:

IFN-γ is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL), effector T cells once antigen-specific immunity develops. IFN-γ stimulates its own expression and up-regulates other genes to stimulate and modulate immunity mainly through the Jak-Stat signalling pathway. It promoters T helper 1 differentiation and cell associated immunity, and also suppresses T helper 2 differentiation and humoral immunity.

IFN-γ is a potent macrophage activator. It binds to macrophage in conjugation with CD40, causing the macrophage to produce elevated amounts ofMHCmolecules. It also stimulates the production of antigen-processing associated transporters and enzymes.

IFN-γ is critical for anti-tumour and anti-viral immunity.

Applications:

ELISA:This antibody can be used as a capture antibody in sandwich ELISA applications for human IFN-γ detection in combination with a monoclonal tracer/detection antibody.

References: If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.