4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:原厂直采,平行进口,授权代理(蚂蚁淘为您服务)
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > cell_proliferation > yesbiotech/mAb抗人MCP-1,S101,检测器/0.1 mg/MO-C40021D
商品详细yesbiotech/mAb抗人MCP-1,S101,检测器/0.1 mg/MO-C40021D
yesbiotech/mAb抗人MCP-1,S101,检测器/0.1 mg/MO-C40021D
yesbiotech/mAb抗人MCP-1,S101,检测器/0.1 mg/MO-C40021D
商品编号: MO-C40021D
品牌: anogen
市场价: ¥4000.00
美元价: 2000.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞增殖
公司分类: cell_proliferation
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
  • Description
  • Additional

Description

Details

Description:Mouse monoclonal antibody to human Monocyte Chemotactic Protein-1 (MCP-1)/ Monocyte Chemotactic and Activating Factor (MCAF)

Purification: Protein G affinity purified

Product Type:Detection antibody in matched antibody pair

Target Protein:Human MCP-1

Immunogen:Purified recombinant human MCP-1

Fusion Myeloma:Sp2/0-Ag14

Specificity:This antibody reacts with natural and recombinant human MCP-1.

Species Reactivity: Human, others not tested.

Cross-reactivity:This antibody does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1β (IL-1β), serum amyloid A (SAA) and epidermal growth factor (EGF).

Host / Isotype: Mouse, IgG1 Kappa

Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20oC

Research Area:Cytokine, chemotaxis, inflammation

Background:

Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displayed chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.

Applications:

ELISA:The antibody can be used for quantitative detection of human MCP-1 in sandwich ELISA in combination with capture antibody S14 (MO-C40021B).

Western Blot:0.1μg/lane of recombinant human MCP-1 can be detected when antibody is used at 0.1-1μg/mL.

Neutralizing:The antibody at a concentration of 0.5μg/mL, neutralized 1nM recombinant human MCP-1 induced monocyte chemotaxis in blind well chemotaxis chambers.

References:

1. Randolph, G. J. and Furie, M. B. A soluble gradient of endogenous monocyte chemoattractant protein-1 promotes the transendothelial migration of monocytes in vitro. J. Immunol. 1995 155:3610-3618.

2. Gwendalyn J. Randolph and Martha B. Furie. Mononuclear phagocytes egress from an in vitro model of the vascular wall by migrating across endothelium in the basal to apical direction: role of intercellular adhesion molecule 1 and the CD11/CD18 integrins. J Exp Med. Feb 1, 1996; 183(2): 451–462.

3. Mehrdad Baghestanian et al. The c-kit Ligand Stem Cell Factor and Anti-IgE Promote Expression of Monocyte Chemoattractant Protein-1 in Human Lung Mast Cells. Blood. December 1, 1997 vol. 90 no. 11 4438-4449.

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.

Additional

Additional Information

Product SpecificitymAb anti-Human MCP-1, S101, Detector
ApplicationELISA, NT, WB
Size0.1 mg
品牌介绍
yesbiotech的自2000年以来,我们已获得ISO认证。我们保证交付给客户的每一种产品都是最优质的。利用我们拥有超过1000个杂交瘤的内部细胞库,我们能够监视和控制整个生产进度,以确保我们的抗体和ELISA试剂盒是市场上最好的。我们为全球主要的生物技术和制药公司,实验室,医院以及学术和政府研究机构提供抗体,ELISA试剂盒和其他实验室试剂。我们还与全球分销商合作,以使我们的产品更容易获得。在Anogen,我们致力于为生物医学研究生产高质量和可靠的抗体产品,并希望我们在该领域的贡献能使患者受益,并改善医学的未来。