Description:Mouse monoclonal antibody (mAb) to human cluster of differentiation 11 alpha chain (CD11α) or mAb to human lymphocyte function associated factor I (LFA-1) alpha chain.

Purification:Protein G affinity purified

Product Type:Primary antibody

Target Protein:Human CD11α

Immunogen:Human cytolytic T lymphocytes

Fusion Myeloma:Sp2/0-Ag14

Specificity:This antibody is reactive to human CD11α.

Species Reactivity:Human, others not tested.

Host / Isotype:Mouse, IgG1 Kappa

Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC

Research Area:Immunology

Background:

CD11α comprises the alpha-chain of lymphocyte function associated factor I (LFA-1). LFA-1 is an adhesion molecule expressed on the surface of T cell, B cell, macrophage, monocytes and neutrophils. LFA-1 plays a role in lymphocyte recruitment to site of infection. LFA-1 initiates a weak link between lymphocytes and antigen presentation cells (APC) by binding to intercellular adhesion molecule 1 (ICAM-1) on APC.

Application:

Fluorescence flow cytometry:Above (see image) is the histogram of Jurkat cells stained with 10mg/ml mouse anti-human CD11α monoclonal antibody. Black line histogram represents Jurkat cells stained with isotype control, mouse anti-TB 38Kd antigen monoclonal antibody (MO-I40021D, B12F8).

Immuno-histochemistry

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.