Description:Mouse monoclonal antibody (mAb) to H. pylori cytotoxin associated gene A (CagA) protein

Purification:Protein G affinity purified

Product Type:Primary antibody, capture antibody in matched antibody pair

Target Protein:H. pylori cytotoxin associated gene A protein

Immunogen:A 32KD recombinant protein cloned from the highly immunogenic region of H. pylori cytotoxin associated gene A.

Fusion Myeloma:Sp2/0-Ag14

Specificity:The mAb reacts with H. pylori CagA

Cross-Reactivity:Cross-reaction with other proteins has not been found.

Host / Isotype:Mouse, IgG2a Kappa

Formulation:Lyophilizedfrom a solution in 0.01M PBS, pH 7.0

Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area:Bacteriology

Background:

Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.

Application:

ELISA: Clone 10E9 coated plate selectively reacts with cell lysate of a CagA containing H. pylori strain whenHRP conjugated anti-CagA clone 3C10 is used as detection antibody. In addition, clone 10E9 coated wells react with recombinant CagA antigen in combination withHRP conjugated anti-CagA mAb clone 5C6, clone 3C10, or clone 3C1.

Western Blot: This clone detects the 32 KD recombinant CagA antigen (immunogen).

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.