INTENDED USE

Pre-titrated Reagent Kit for human IL-8 contains calibrated IL-8 standards and pre-titrated capture and detection antibodies for quantitative measurement of human IL-8 in biological samples by enzyme-linked immunoassay (ELISA). An assay protocol is provided with the kit to use the reagents in 96-well ELISA format. It is recommended to dilute all reagents according to this manual. The lyophilized standards have been calibrated against WHO reference material to ensure accuracy and consistence of test results. The reagents can also be used with 384-well ELISA plate and optimized for use in other type of immunoassays.

Background

Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8"s primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.