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当前位置: 首页 > 产品中心 > peptide > yesbiotech/人MCP-3 ELISA试剂盒/96孔/EL10016
商品详细yesbiotech/人MCP-3 ELISA试剂盒/96孔/EL10016
yesbiotech/人MCP-3 ELISA试剂盒/96孔/EL10016
yesbiotech/人MCP-3 ELISA试剂盒/96孔/EL10016
商品编号: EL10016
品牌: anogen
市场价: ¥8100.00
美元价: 4050.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
  • Description
  • Additional

Description

Details

REACTIVITY

Human

SENSITIVITY

<30 pg/mL

ASSAY RANGE

30-1600 pg/mL

REAGENTS PROVIDED

MCP-3 MICROTITER PLATEMCP-3CONJUGATEMCP-3STANDARDCALIBRATOR DILUENT ICALIBRATOR DILUENT IIWASH BUFFER (20X/96 wells, 30X/192 wells)SUBSTRATE ASUBSTRATE BSTOP SOLUTION

INTENDED USE

This Human MCP-3 ELISA Kit is to be used for the in vitro quantitative determination of human monocyte chemotactic protein-3 (MCP-3) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.

INTRODUCTION

Monocyte Chemotactic Protein (MCP-3) is a novel chemokine that has been recently purified from human osteosarcoma cell line1. It was shown that MCP-3 is produced by both tumours cells1 and leukocytes.2,3 MCP-3 can bind and activate a vast diversity of inflammatory cell types through interaction with multiple leukocyte receptors as well as it’s own receptor.6,7 Murine MCP‑3 Marc/Fic chemokines that have been previously cloned are thought to be homologues of human MCP-3.3,4 MCP-3 cDNA cloning2 and structural analysis revealed that this 76 amino acid (a.a.) polypeptide with a molar mass of 8.5 kDa belongs to a family of small inflammatory proteins, characterized by four conserved cysteine residues and is localized on human chromosome 17.1,4,5 MCP-3 is designated a C-C or intercrine ß cytokine.13 MCP-3 can indeed use a wide variety of binding sites and activate many inflammatory cells. In vitro, both MCP-3 and MCP-1 can activate T-cells, monocytes, and basophils, but only the first can activate eosinophils. These cytokines show 71% a.a. homology. MCP‑3 was also revealed to have 30% a.a homology with MIP-1 and RANTES which can also activate eosinophils plus all cell types mentioned above.6,7 MCP-3 seems to be the only C-Cchemokine that regularly induces neutrophil migration. It is also a potent chemoattractant for human dendritic cells.7

It has been suggested that MCP-3 binds multiple C-C receptors such as MCP-1 on monocytes and basophils 8,9, MIP-1a on neutrophils, basophils, and eosinophils9 and RANTES on basophils and eosinophils(9,10). Evidence suggests that MCP-3 does indeed use multiple receptors (MCP-1, RANTES, and MIP-1a) and binds with MIP-1ß receptor as well as other unique binding sites 6,7.MCP-3 was discovered to be the strongest C‑Cchemokine in inducing the migration of C-C CKR1 transfected cells. It was shown that MCP-3 binds with C-C CKR1 receptor with greater affinity than MIP‑1a or Rantes, which mainly activate it 6,11. Also, MCP-3 promotes exocytosis of eosinophil granule proteins and stimulates histamine release from human basophils 4,12. In vivo, MCP-3 induces the selective infiltration of monocytes on intradermal injection in rabbits.1 Since MCP-3 acts on a variety of inflammatory cells and utilizes multiple receptors for its function, characterization and isolation of the shared as well as unique receptors for MCP-3 will provide further insights into the pathophysiological roles of MCP-3. C-C chemokines are mediators of a number of pathological conditions such as chronic inflammation, tumor, allergy, as well as atherosclerosis10. Since the binding and signaling of MCP-3 is most promiscuous, the development of antibody or antagonist which can block MCP-3 through binding to the receptor or ligand may prove to be useful in the treatment of diseases mediated by a number of C-C chemokine.

This MCP-3 ELISA is a 2.5 hour solid phase immunoassay readily applicable to measure MCP-3 in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 1600 pg/mL. It showed no cross-reactivity with other cytokines tested. MCP-3 may play a role in certain diseases, therefore this MCP-3 ELISA is expected to be effectively used for further investigations into the relationship between MCP-3 and various pathological conditions.

Additional

Additional Information

Product SpecificityHuman MCP-3 ELISA Kit
ApplicationRefer to Insert
Size96 wells
品牌介绍
yesbiotech的自2000年以来,我们已获得ISO认证。我们保证交付给客户的每一种产品都是最优质的。利用我们拥有超过1000个杂交瘤的内部细胞库,我们能够监视和控制整个生产进度,以确保我们的抗体和ELISA试剂盒是市场上最好的。我们为全球主要的生物技术和制药公司,实验室,医院以及学术和政府研究机构提供抗体,ELISA试剂盒和其他实验室试剂。我们还与全球分销商合作,以使我们的产品更容易获得。在Anogen,我们致力于为生物医学研究生产高质量和可靠的抗体产品,并希望我们在该领域的贡献能使患者受益,并改善医学的未来。